This volume covers a broad range of procedures and protocols that discuss the steps for designing experimental setups for studying sperm, including analytical tools for diverse species. The chapters in this book are organized into 5 parts: Part One looks at the latest classical methods of analysis of sperm quality parameters; Part Two discusses the process of sperm collection in wild and domestic species; Part Three covers spermatozoa, including cryopreservation and analysis of a variety of sperm characteristics; Part Four focuses on molecular determinants in seminal plasma; and Part Five talks about sperm interactions, describing the fine cross-talk of the male gametes with the female genital tract. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Spermatology: Methods and Protocols is a valuable resource for both novel and expert researchers who want to learn more about sperm-related methodologies.