Therapeutic gardens are a very old practice in the area of health and, nowadays, they gained strength from the discussion about how the connection with nature can influence human well-being, in addition to the pressing concern for the environment that surrounds us, which includes health services, which are intended for the recovery, prevention and/or promotion of healthy states for all people who circulate there, not just patients. Brazil is one of the countries with the greatest biodiversity in the world. The implementation of therapeutic gardens is an action that promotes environmental, social and economic benefits, and thus contributes to the sustainable agenda. This guide, therefore, is an indispensable resource for professionals in different areas of knowledge, such as architects, biologists, botanists, landscapers, health designers, managers of institutions at all levels of health care, therapists, clinicians and other publics interested in health and nature.