Nanotechnology is the study of the nanoscale: objects around a nanometer (NM) in size. Our ability to develop enormous, complex products with nanometer accuracy is quickly evolving, and comprises of hierarchical reductive methodologies and base up additive approaches. On the other hand, nature has idealized a variety of organic tools that operate at the nanoscale, structures which regularly self-regulate driven by the atomic science of subunit combinations. This chapter describes various terminologies in nanofabrication and organic gathering, microscopy, and nanoscale natural systems. Nanotechnology includes the advancement of manmade or artificial particles and sub-atomic particles that have aspects in the nanometer range (somewhere in the range of 1 and 100 nm in some respect). When combined with biotechnology, nanotechnology becomes an incredible new stage with countless applications across a wide number of pragmatic applications including farming, indicative technology, new drugs, clinical imaging, biological sensors, and others. Currently, the quantity of possible types of nanomaterials accessible for use in biotechnological applications includes nanoparticles, nanowires, nanofibers, nanostructures, and nanomachines. The risk potential of nanomaterials is however arising, and showing that there are a few materials that have qualities that might require some level of upgrade to eliminate the chance of unfavorable impacts and an open risk. In spite of the difficulties, the commercialization of nanobiotechnology products seems to have a splendid future, and within 10 years, numerous new results of this nature are probably going to be supported and used across different sectors.